Coding

Part:BBa_K1690005:Design

Designed by: Anna Sosnova, Pavel Zach   Group: iGEM15_Czech_Republic   (2015-09-17)


Pheromone receptor (C. parapsilosis)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 280
    Illegal BglII site found at 1085
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 340
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The coding sequence was codon optimized for the expression in S. cerevisiae. Kozak sequence was added before the start codon for a better translation. C-terminal was replaced by a C-terminal from C. albicans for more efficient coupling to the mating pathway in S. cerevisiae.


Source

The whole sequence was synthetized.

References